My Lab Notebook

Reverse Transcription Began Transcription of the RNA samples. Here is the protocol. Sam walked me through it last week. Completed: [Read More]

plotting some growth. here is the code ```{r} #retrieve csv size_data <- read.csv(“~/project-gigas-carryover/lifestage_carryover/data/size/gigas-lengths.csv”) #filtering/grouping i_treatment_data <- size_data[size_data$init.treat == “treatment”, ] i_control_data <- size_data[size_data$init.treat == “control”, ] #getting R to read dates as dates i_treatment_data$dates <- as.Date(i_treatment_data$Date, format= “%m/%d/%Y”) i_control_data$dates <- as.Date(i_control_data$Date, format= “%m/%d/%Y”) size_data$dates <- as.Date(size_data$Date, format= “%m/%d/%Y”) #specifying the... [Read More]

lengths spreadsheet. Averages are colored orange. [Read More]

Organization github repo lengths spreadsheet 1st sampling / RNA extractions spreadsheet [Read More]

Project Thinking Advice from Ariana: Should start doing random extractions instead of just going down the spreadhseet to avoid possible complications (e.g what if bad reagent and now a whole group is unknowable). Might also take notes of the reagents used and their dates. Would love to do a new... [Read More]